PGP 9.5 Antibody

NSJ Bioreagents
Product Code: NSJ-F47953
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F47953-0.08ML0.08 ml£260.00
Quantity:
NSJ-F47953-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the PGP 9.5 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 5
PGP 9.5 antibody western blot analysis in (1) U266, (2) NCI-H1299, (3) mouse Neuro-2a cell line and (4) mouse brain tissue lysate. Predicted molecular weight ~25 kDa.
2 / 5
Western blot analysis of lysate from (1) SH-SY5Y cell line and (2) mouse brain tissue using PGP 9.5 antibody at 1:1000. Predicted molecular weight ~25 kDa.
3 / 5
Fluorescent image of U251 cell stained with PGP 9.5 antibody at 1:25. Immunoreactivity is localized to the cytoplasm and nucleus.
4 / 5
PGP 9.5 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue.
5 / 5
PGP 9.5 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human cerebellum tissue.

PGP 9.5 antibody western blot analysis in (1) U266, (2) NCI-H1299, (3) mouse Neuro-2a cell line and (4) mouse brain tissue lysate. Predicted molecular weight ~25 kDa.
Western blot analysis of lysate from (1) SH-SY5Y cell line and (2) mouse brain tissue using PGP 9.5 antibody at 1:1000. Predicted molecular weight ~25 kDa.
Fluorescent image of U251 cell stained with PGP 9.5 antibody at 1:25. Immunoreactivity is localized to the cytoplasm and nucleus.
PGP 9.5 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue.
PGP 9.5 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human cerebellum tissue.

Further Information

Application Details :
Western blot: 1:1000,Immunofluorescence: 1:10-1:50,IHC (Paraffin): 1:10-1:50
Application Note:
Titration of the PGP 9.5 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
UCHL1 is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. Expression of UCHL1 is highly specific to neurons and to cells of the diffuse neuroendocrine system and their tumors. It is present in all neurons.
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 187-214 from the human protein was used as the immunogen for this PGP 9.5 antibody.
Limitation:
This PGP 9.5 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Rat
Species Reactivity (Predicted):
Mouse, Bovine, Pig, Primate
Uniprot #:
P09936