Phospho-p27Kip1 Antibody (pT197)

NSJ Bioreagents
Product Code: NSJ-F48820
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F48820-0.08ML0.08 ml£260.00
Quantity:
NSJ-F48820-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Dot Blot
  • Enzyme-Linked Immunosorbent Assay (ELISA)
Storage:
Aliquot the phospho-p27Kip1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Dot blot analysis of phospho-p27Kip1 antibody. 50ng of phos-peptide or nonphos-peptide per dot were spotted.

Dot blot analysis of phospho-p27Kip1 antibody. 50ng of phos-peptide or nonphos-peptide per dot were spotted.

Further Information

Application Details :
Dot blot: 1:500
Application Note:
Titration of the phospho-p27Kip1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. [provided by RefSeq].
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
This phospho-p27Kip1 antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pT197 of mouse p27Kip1.
Limitation:
This phospho-p27Kip1 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Mouse
Uniprot #:
P46414