Anti-GST Antibody

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NSJ Bioreagents
Product Code: NSJ-F52068
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F52068-0.08ML0.08 ml£260.00
Quantity:
NSJ-F52068-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot the anti-GST antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Anti-GST antibody used in western blot to detect a GST-tagged recombinant protein purified from bacterial lysate (Lanes 1-3: 10, 40, and 160 ng protein).
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Anti-GST antibody used in western blot to detect a GST-tagged recombinant protein purified from bacterial lysate (Lanes 1-3: 10, 40, and 160 ng protein).

Documents

Further Information

Application Details :
Western blot: 1:1000
Application Note:
Titration of the anti-GST antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Glutathione S-transferase (GST) was originally cloned from parasite Schistosoma japonicum and it is now a widely used protein fusion partner. Vectors containing GST Tag have been developed for both prokaryotic and eukaryotic systems. The GST fusion proteins are easily purified from cell lysates by affinity chromatography using Glutathione Sepharose 4B to elute out the GST fusion protein from the column with a denaturing form of glutathione. Using the NSJBio anti-GST antibody provides a simple solution to detect the expression of GST fusion proteins in cells.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
Recombinant full length GST was used as the immunogen for this anti-GST antibody.
Limitation:
This anti-GST antibody is available for research use only.
Purity:
Purified