FLAG Antibody

NSJ Bioreagents
Product Code: NSJ-F52063
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F52063-0.05ML0.05 ml£260.00
Quantity:
NSJ-F52063-0.2ML0.2 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot the FLAG antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot analysis of lysate from a 41 kDa 12 tagged protein using FLAG antibody at 1:1000.

Western blot analysis of lysate from a 41 kDa 12 tagged protein using FLAG antibody at 1:1000.

Further Information

Application Details :
Western blot: 1:1000
Application Note:
Titration of the FLAG antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Epitope tags are useful for the labeling and detection of recombinant proteins using western blotting, immunoprecipitation and immunostaining techniques. The eight amino acid DYKDDDDK peptide is an established and multi-functional epitope tag and can be expressed and detected with a recombinant protein as an amino-terminal or carboxy-terminal fusion.
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
The sequence cDYKDDDDK was used as the immunogen for this FLAG antibody.
Limitation:
This FLAG antibody is available for research use only.
Purity:
Antigen affinity