HA Antibody

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NSJ Bioreagents
Product Code: NSJ-F52062
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F52062-0.05ML0.05 ml£260.00
Quantity:
NSJ-F52062-0.2ML0.2 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • ChIP
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Aliquot the HA antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot analysis of lysate from 12 tagged recombinant protein cell lines using HA antibody diluted at 1:1000.
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Western blot analysis of HA antibody and tagged recombinant protein bacterial lysate
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GTPase activity of Dynamin-2 is required for endocytosis of cell-surface tTG. MRC-5 fibroblasts were transiently transfected with either wild-type (wt) or a GTPase-deficient Dynamin-2 mutant with a hemagglutinin (HA) tag.(Provided by Evgeny A. Zemskov, Irina Mikhailenko)
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Western blot analysis of lysate from 12 tagged recombinant protein cell lines using HA antibody diluted at 1:1000.
Western blot analysis of HA antibody and tagged recombinant protein bacterial lysate
GTPase activity of Dynamin-2 is required for endocytosis of cell-surface tTG. MRC-5 fibroblasts were transiently transfected with either wild-type (wt) or a GTPase-deficient Dynamin-2 mutant with a hemagglutinin (HA) tag.(Provided by Evgeny A. Zemskov, Irina Mikhailenko)

Documents

Further Information

Application Details :
Western blot: 1:1000,Immunofluorescence: 1:10-1:50,ChIP: 1:100
Application Note:
Titration of the HA antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Epitope tags consisting of short sequences recognized by well-characterizated antibodies have been widely used in the study of protein expression in various systems. The HA tag (YPYDVPDYAYPYDVPDYA) is an established example. NSJBio's anti-HA polyclonal antibody provides a simple solution to detect the expression of an HA-tagged protein in cells.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
The sequence CYPYDVPDYAYPYDVPDYA was used as the immunogen for this HA antibody.
Limitation:
This HA antibody is available for research use only.
Purity:
Purified