SPHK1 Antibody

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NSJ Bioreagents
Product Code: NSJ-F50217
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F50217-0.05ML0.05 ml£260.00
Quantity:
NSJ-F50217-0.2ML0.2 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Non-Human Primates
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the SPHK1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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IHC analysis of FFPE human hepatocarcinoma tissue stained with the SPHK1 antibody
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Western blot analysis of lysate from 1) 293, 2) HepG2, 3) HUVEC, 4) Raji, 5) rat C6 and 6) mouse C2C12 cell line using SPHK1 antibody at 1:1000. Predicted molecular weight: 42-51 kDa.
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The SPHK1 antibody used in western blot to detect SPHK1 in mouse kidney tissue lysate
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Western blot analysis of SPHK1 antibody and 293 cell lysate either nontransfected (Lane 1) or transiently transfected (2) with the SPHK1 gene.
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IHC analysis of FFPE human heart tissue stained with the SPHK1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
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IHC analysis of FFPE human hepatocarcinoma tissue stained with the SPHK1 antibody
Western blot analysis of lysate from 1) 293, 2) HepG2, 3) HUVEC, 4) Raji, 5) rat C6 and 6) mouse C2C12 cell line using SPHK1 antibody at 1:1000. Predicted molecular weight: 42-51 kDa.
The SPHK1 antibody used in western blot to detect SPHK1 in mouse kidney tissue lysate
Western blot analysis of SPHK1 antibody and 293 cell lysate either nontransfected (Lane 1) or transiently transfected (2) with the SPHK1 gene.
IHC analysis of FFPE human heart tissue stained with the SPHK1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.

Documents

Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:50-1:100
Application Note:
Titration of the SPHK1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Sphingosine Kinase (SphK) catalyzes the phosphorylation of the lipid sphingosine, creating the bioactive lipid sphingosine-1-phosphate (S1P). S1P subsequently signals through cell surface G protein-coupled receptors, as well as intracellularly, to modulate cell proliferation, survival, motility and differentiation. SphK is an important signaling enzyme which is activated by diverse agents, including growth factors that signal through receptor tyrosine kinases, agents activating G protein-coupled receptors, and immunoglobulin receptors. Two SphK isotypes, SphK-1 and SphK-2, have been cloned, and both isotypes are ubiquitously expressed. SphK-1 has been shown to mediate cell growth, prevention of apoptosis, and cellular transformation, and is upregulated in a variety of human tumors. In contrast, SphK-2 increases apoptosis, and may be responsible for phosphorylating and activating the immunosuppressive drug FTY720.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 286-315 from the human protein was used as the immunogen for this SPHK1 antibody.
Limitation:
This SPHK1 antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human, Mouse, Rat, Primate
Uniprot #:
Q9NYA1