p62 Antibody

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NSJ Bioreagents
Product Code: NSJ-F48010
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F48010-0.08ML0.08 ml£260.00
Quantity:
NSJ-F48010-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • African Green Monkey (sabaeus)
  • Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the p62 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot testing of p62 antibody at 1:500 dilution + HeLa lysate; Predicted band size : 47~62 kDa.
2 / 6
Fluorescent image of U251 cells stained with p62 antibody at 1:200. Immunoreactivity is localized to autophagic vacuoles in the cytoplasm.
3 / 6
Western blot analysis of p62 antibody pre-incubated without (Lane 1) and with (2) blocking peptide in MCF-7 lysate
4 / 6
Western blot analysis of p6 antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SQSTM1/p62 gene (2).
5 / 6
IHC analysis of FFPE human hepatocarcinoma tissue stained with the p62 antibody
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Immunofluorescence staining of p62 antibody on Methanol-fixed and PFA fixed HeLa cells. Data courtesy of Dr. Eeva-Liisa Eskelinen, University of Helsinki, Finland.
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Western blot testing of p62 antibody at 1:500 dilution + HeLa lysate; Predicted band size : 47~62 kDa.
Fluorescent image of U251 cells stained with p62 antibody at 1:200. Immunoreactivity is localized to autophagic vacuoles in the cytoplasm.
Western blot analysis of p62 antibody pre-incubated without (Lane 1) and with (2) blocking peptide in MCF-7 lysate
Western blot analysis of p6 antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SQSTM1/p62 gene (2).
IHC analysis of FFPE human hepatocarcinoma tissue stained with the p62 antibody
Immunofluorescence staining of p62 antibody on Methanol-fixed and PFA fixed HeLa cells. Data courtesy of Dr. Eeva-Liisa Eskelinen, University of Helsinki, Finland.

Documents

Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:50-1:100,Immunofluorescence: 1:50-1:100
Application Note:
Titration of the p62 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
SQSTM1/p62 is an adapter protein which binds ubiquitin and may regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. This protein may play a role in titin/TTN downstream signaling in muscle cells, and may also regulate signaling cascades through ubiquitination. This protein is involved in cell differentiation, apoptosis, immune response and regulation of K(+) channels. SQSTM1/p62 also appears to play a role in macroautophagic removal of intracellular protein aggregates. Cellular depletion studies of SQSTM1/p62 have indicated a role for association with LC3 and aggregate proteins in order to facilitate normal formation of the autophagosome.
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 317-346 from the human protein was used as the immunogen for this p62 antibody.
Limitation:
This p62 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, African Green Monkey
Species Reactivity (Predicted):
Mouse, Rat
Uniprot #:
Q13501