cKit Antibody

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NSJ Bioreagents
Product Code: NSJ-F40156
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F40156-0.08ML0.08 ml£260.00
Quantity:
NSJ-F40156-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Non-Human Primates
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot the cKit antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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cKit antibody used in western blot to detect KIT in serum-starved HeLa cell lysate (lane 1) and primate testis tissue lysate (2).
2 / 3
cKit antibody was used in immunohistochemistry on breast carcinoma.
3 / 3
Flow cytometric analysis of HeLa cells using cKit antibody (green) compared to a negative control cell (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
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cKit antibody used in western blot to detect KIT in serum-starved HeLa cell lysate (lane 1) and primate testis tissue lysate (2).
cKit antibody was used in immunohistochemistry on breast carcinoma.
Flow cytometric analysis of HeLa cells using cKit antibody (green) compared to a negative control cell (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.

Documents

Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Flow Cytometry: 1:10-1:50
Application Note:
Titration of the cKit antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
c-Kit is a Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. [UniProt]
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 9-40 from the human protein was used as the immunogen for this cKit antibody.
Limitation:
This cKit antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human, Primate
Uniprot #:
P10721