TIMP1 Antibody

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NSJ Bioreagents
Product Code: NSJ-F54350
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54350-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54350-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the TIMP-1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 5
Immunofluorescent staining of fixed and permeabilized human A2058 cells with TIMP-1 antibody (green) and anti-Actin (red).
2 / 5
Immunofluorescent staining of fixed and permeabilized human A2058 cells with TIMP-1 antibody (green) and DAPI nuclear stain (blue).
3 / 5
IHC testing of FFPE human pancreas tissue with TIMP-1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
4 / 5
Western blot testing of human CCRF-CEM cell lysate with TIMP-1 antibody. Expected molecular weight: 23-28 kDa depending on the level of glycosylation.
5 / 5
Flow cytometry testing of fixed and permeabilized human A2058 cells with TIMP-1 antibody; Blue=isotype control, Green= TIMP-1 antibody.
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Immunofluorescent staining of fixed and permeabilized human A2058 cells with TIMP-1 antibody (green) and anti-Actin (red).
Immunofluorescent staining of fixed and permeabilized human A2058 cells with TIMP-1 antibody (green) and DAPI nuclear stain (blue).
IHC testing of FFPE human pancreas tissue with TIMP-1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of human CCRF-CEM cell lysate with TIMP-1 antibody. Expected molecular weight: 23-28 kDa depending on the level of glycosylation.
Flow cytometry testing of fixed and permeabilized human A2058 cells with TIMP-1 antibody; Blue=isotype control, Green= TIMP-1 antibody.

Documents

Further Information

Application Details :
Flow cytometry: 1:25 (1x10e6 cells),Immunofluorescence: 1:25,Western blot: 1:500-1:2000,Immunohistochemistry (FFPE): 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the TIMP-1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This gene belongs to the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix. In addition to its inhibitory role against most of the known MMPs, the encoded protein is able to promote cell proliferation in a wide range of cell types, and may also have an anti-apoptotic function. Transcription of this gene is highly inducible in response to many cytokines and hormones. In addition, the expression from some but not all inactive X chromosomes suggests that this gene inactivation is polymorphic in human females. This gene is located within intron 6 of the synapsin I gene and is transcribed in the opposite direction.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 157-188 from the human protein was used as the immunogen for the TIMP-1 antibody.
Limitation:
This TIMP-1 antibody is available for research use only.
Purity:
SAS precipitation
Species Reactivity :
Human
Uniprot #:
P01033