Beta Arrestin 1 Antibody / ARRB1

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NSJ Bioreagents
Product Code: NSJ-F54630
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54630-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54630-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the ARRB1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot testing of human A549 cell lysate with ARRB1 antibody. Predicted molecular weight ~47 kDa.
2 / 4
IHC testing of FFPE human brain tissue with ARRB1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
3 / 4
Immunofluorescent staining of human A549 cells with ARRB1 antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
4 / 4
Flow cytometry testing of human A549 cells with ARRB1 antibody; Blue=isotype control, Green= ARRB1 antibody.
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Western blot testing of human A549 cell lysate with ARRB1 antibody. Predicted molecular weight ~47 kDa.
IHC testing of FFPE human brain tissue with ARRB1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of human A549 cells with ARRB1 antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
Flow cytometry testing of human A549 cells with ARRB1 antibody; Blue=isotype control, Green= ARRB1 antibody.

Documents

Further Information

Application Details :
Flow cytometry: 1:25 (1x10e6 cells), Immunofluorescence: 1:25, Immunohistochemistry (FFPE): 1:25, Western blot: 1:500-1:2000
Application Note:
The stated application concentrations are suggested starting points. Titration of the ARRB1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Members of arrestin/beta-arrestin protein family are thought to participate in agonist-mediated desensitization of G-protein-coupled receptors and cause specific dampening of cellular responses to stimuli such as hormones, neurotransmitters, or sensory signals. Arrestin beta 1 is a cytosolic protein and acts as a cofactor in the beta-adrenergic receptor kinase (BARK) mediated desensitization of beta-adrenergic receptors. Besides the central nervous system, it is expressed at high levels in peripheral blood leukocytes, and thus the BARK/beta-arrestin system is believed to play a major role in regulating receptor-mediated immune functions. Alternatively spliced transcripts encoding different isoforms of arrestin beta 1 have been described, however, their exact functions are not known.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 336-363 from the human protein was used as the immunogen for the ARRB1 antibody.
Limitation:
This ARRB1 antibody is available for research use only.
Localization:
Cytoplasmic, nuclear
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
P49407