Nix Antibody / BNIP3L

NSJ Bioreagents
Product Code: NSJ-F54613
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54613-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54613-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the BNIP3L antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
IHC testing of FFPE human uterus tissue with BNIP3L antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 4
Immunofluorescent staining of human A549 cells with BNIP3L antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
3 / 4
Western blot testing of human A549 cell lysate with BNIP3L antibody. Expected molecular weight: ~38 kDa (monomer), ~76 kDa (homodimer).
4 / 4
Flow cytometry testing of human A549 cells with BNIP3L antibody; Blue=isotype control, Green= BNIP3L antibody.

IHC testing of FFPE human uterus tissue with BNIP3L antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of human A549 cells with BNIP3L antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
Western blot testing of human A549 cell lysate with BNIP3L antibody. Expected molecular weight: ~38 kDa (monomer), ~76 kDa (homodimer).
Flow cytometry testing of human A549 cells with BNIP3L antibody; Blue=isotype control, Green= BNIP3L antibody.

Further Information

Application Details :
Flow cytometry: 1:25 (1x10e6 cells), Immunofluorescence: 1:25, Immunohistochemistry (FFPE): 1:25, Western blot: 1:500-1:2000
Application Note:
The stated application concentrations are suggested starting points. Titration of the BNIP3L antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This gene is a member of the BCL2/adenovirus E1B 19 kd-interacting protein (BNIP) family. It interacts with the E1B 19 kDa protein which is responsible for the protection of virally-induced cell death, as well as E1B 19 kDa-like sequences of BCL2, also an apoptotic protector. The protein encoded by this gene is a functional homolog of BNIP3, a proapoptotic protein. This protein may function simultaneously with BNIP3 and may play a role in tumor suppression.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 52-81 from the human protein was used as the immunogen for the BNIP3L antibody.
Limitation:
This BNIP3L antibody is available for research use only.
Localization:
Cytoplasmic, nuclear
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
O60238