Chromogranin A Antibody

NSJ Bioreagents
Product Code: NSJ-F54612
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54612-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54612-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the CMGA antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Western blot testing of human A549 cell lysate with CMGA antibody. Expected molecular weight: 50-75 kDa depending on glycosylation level.
2 / 4
IHC testing of FFPE human pancreas tissue with CMGA antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
3 / 4
Immunofluorescent staining of human A549 cells with CMGA antibody (green) and DAPI nuclear stain (blue).
4 / 4
Flow cytometry testing of human A549 cells with CMGA antibody; Blue=isotype control, Green= CMGA antibody.

Western blot testing of human A549 cell lysate with CMGA antibody. Expected molecular weight: 50-75 kDa depending on glycosylation level.
IHC testing of FFPE human pancreas tissue with CMGA antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of human A549 cells with CMGA antibody (green) and DAPI nuclear stain (blue).
Flow cytometry testing of human A549 cells with CMGA antibody; Blue=isotype control, Green= CMGA antibody.

Further Information

Application Details :
Flow cytometry: 1:25 (1x10e6 cells), Immunofluorescence: 1:25, Immunohistochemistry (FFPE): 1:25, Western blot: 1:500-1:2000
Application Note:
The stated application concentrations are suggested starting points. Titration of the CMGA antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
The protein encoded by this gene is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is found in secretory vesicles of neurons and endocrine cells. This gene product is a precursor to three biologically active peptides; vasostatin, pancreastatin, and parastatin. These peptides act as autocrine or paracrine negative modulators of the neuroendocrine system. Other peptides, including chromostatin, beta-granin, WE-14 and GE-25, are also derived from the full-length protein. However, biological activities for these molecules have not been shown.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 376-404 from the human protein was used as the immunogen for the CMGA antibody.
Limitation:
This CMGA antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
P10645