Galactosidase alpha Antibody / Gla

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NSJ Bioreagents
Product Code: NSJ-F54879
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54879-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54879-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Galactosidase alpha antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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IHC testing of FFPE human lung carcinoma tissue with Galactosidase alpha antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 4
Immunofluorescent staining of human HeLa cells with Galactosidase alpha antibody (green) and DAPI nuclear stain (blue).
3 / 4
Flow cytometry testing of human HepG2 cells with Galactosidase alpha antibody; Blue=isotype control, Green= Galactosidase alpha antibody.
4 / 4
Western blot testing of human HeLa cell lysate with Galactosidase alpha antibody. Predicted molecular weight ~48 kDa.
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IHC testing of FFPE human lung carcinoma tissue with Galactosidase alpha antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of human HeLa cells with Galactosidase alpha antibody (green) and DAPI nuclear stain (blue).
Flow cytometry testing of human HepG2 cells with Galactosidase alpha antibody; Blue=isotype control, Green= Galactosidase alpha antibody.
Western blot testing of human HeLa cell lysate with Galactosidase alpha antibody. Predicted molecular weight ~48 kDa.

Documents

Further Information

Application Details :
Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunofluorescence: 1:10-1:50, Immunohistochemistry (FFPE): 1:10-1:50, Western blot: 1:500-1:1000
Application Note:
The stated application concentrations are suggested starting points. Titration of the Galactosidase alpha antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
GLA is a homodimeric glycoprotein that hydrolyses the terminal alpha-galactosyl moieties from glycolipids and glycoproteins. This enzyme predominantly hydrolyzes ceramide trihexoside, and it can catalyze the hydrolysis of melibiose into galactose and glucose. A variety of mutations in this gene affect the synthesis, processing, and stability of this enzyme, which causes Fabry disease, a rare lysosomal storage disorder that results from a failure to catabolize alpha-D-galactosyl glycolipid moieties.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 83-112 from the human protein was used as the immunogen for the Galactosidase alpha antibody.
Limitation:
This Galactosidase alpha antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Purified
Species Reactivity :
Human
Uniprot #:
P06280