Gastric Intrinsic Factor Antibody / GIF / CBLIF

NSJ Bioreagents
Product Code: NSJ-F54661
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54661-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54661-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Gastric Intrinsic Factor antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Western blot testing of human K562 cell lysate with Gastric Intrinsic Factor antibody. Predicted molecular weight ~45 kDa.
2 / 4
IHC testing of FFPE human stomach tissue with Gastric Intrinsic Factor antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
3 / 4
Immunofluorescent staining of human HEK293 cells with Gastric Intrinsic Factor antibody (green) and DAPI nuclear stain (blue).
4 / 4
Flow cytometry testing of human K562 cells with Gastric Intrinsic Factor antibody; Blue=isotype control, Green= Gastric Intrinsic Factor antibody.

Western blot testing of human K562 cell lysate with Gastric Intrinsic Factor antibody. Predicted molecular weight ~45 kDa.
IHC testing of FFPE human stomach tissue with Gastric Intrinsic Factor antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of human HEK293 cells with Gastric Intrinsic Factor antibody (green) and DAPI nuclear stain (blue).
Flow cytometry testing of human K562 cells with Gastric Intrinsic Factor antibody; Blue=isotype control, Green= Gastric Intrinsic Factor antibody.

Further Information

Application Details :
Flow cytometry: 1:25 (1x10e6 cells), Immunofluorescence: 1:25, Immunohistochemistry (FFPE): 1:25, Western blot: 1:500-1:2000
Application Note:
The stated application concentrations are suggested starting points. Titration of the Gastric Intrinsic Factor antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This gene is a member of the cobalamin transport protein family. It encodes a glycoprotein secreted by parietal cells of the gastric mucosa and is required for adequate absorption of vitamin B12. Vitamin B12 is necessary for erythrocyte maturation and mutations in this gene may lead to congenital pernicious anemia.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 189-218 from the human protein was used as the immunogen for the Gastric Intrinsic Factor antibody.
Limitation:
This Gastric Intrinsic Factor antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
P27352