IL1RN Antibody / IL1RA / Interleukin-1 Receptor Antagonist

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NSJ Bioreagents
Product Code: NSJ-F54851
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54851-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54851-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the IL1RN antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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IHC testing of FFPE human colon carcinoma tissue with IL1RN antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 4
Immunofluorescent staining of human WiDr cells with IL1RN antibody (green) and DAPI nuclear stain (blue).
3 / 4
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with IL1RN antibody.
4 / 4
Flow cytometry testing of human HEK293 cells with IL1RN antibody; Blue=isotype control, Green= IL1RN antibody.
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IHC testing of FFPE human colon carcinoma tissue with IL1RN antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of human WiDr cells with IL1RN antibody (green) and DAPI nuclear stain (blue).
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with IL1RN antibody.
Flow cytometry testing of human HEK293 cells with IL1RN antibody; Blue=isotype control, Green= IL1RN antibody.

Documents

Further Information

Application Details :
Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunofluorescence: 1:10-1:50, Immunohistochemistry (FFPE): 1:10-1:50, Western blot: 1:500-1:1000
Application Note:
The stated application concentrations are suggested starting points. Titration of the IL1RN antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
The protein is a member of the interleukin 1 cytokine family. This protein inhibits the activities of interleukin 1, alpha (IL1A) and interleukin 1, beta (IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses. A polymorphism of its gene is reported to be associated with increased risk of osteoporotic fractures and gastric cancer.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 100-129 from the human protein was used as the immunogen for the IL1RN antibody.
Limitation:
This IL1RN antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Purified
Species Reactivity :
Human
Uniprot #:
P18510