MBD3L3 Antibody

NSJ Bioreagents
Product Code: NSJ-F54637
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54637-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54637-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the MBD3L3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Western blot testing of human MCF7 cell lysate with MBD3L3 antibody. Predicted molecular weight ~23 kDa.
2 / 4
IHC testing of FFPE human testis carcinoma tissue with MBD3L3 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
3 / 4
Immunofluorescent staining of human MCF7 cells with MBD3L3 antibody (green) and DAPI nuclear stain (blue).
4 / 4
Flow cytometry testing of human MCF7 cells with MBD3L3 antibody; Blue=isotype control, Green= MBD3L3 antibody.

Western blot testing of human MCF7 cell lysate with MBD3L3 antibody. Predicted molecular weight ~23 kDa.
IHC testing of FFPE human testis carcinoma tissue with MBD3L3 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of human MCF7 cells with MBD3L3 antibody (green) and DAPI nuclear stain (blue).
Flow cytometry testing of human MCF7 cells with MBD3L3 antibody; Blue=isotype control, Green= MBD3L3 antibody.

Further Information

Application Details :
Flow cytometry: 1:25 (1x10e6 cells), Immunofluorescence: 1:25, Immunohistochemistry (FFPE): 1:25, Western blot: 1:500-1:2000
Application Note:
The stated application concentrations are suggested starting points. Titration of the MBD3L3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
The MBD3L proteins are encoded by strongly repeated regions of the 19p13 chromosome. The exact number of functional copies is unclear, and some of them may represent pseudogenes. [UniProt]
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 156-184 from the human protein was used as the immunogen for the MBD3L3 antibody.
Limitation:
This MBD3L3 antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
A6NE82