NKD2 Antibody / Naked-2

NSJ Bioreagents
Product Code: NSJ-F54639
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54639-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54639-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the NKD2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Western blot testing of human Jurkat cell lysate with NKD2 antibody. Expected molecular weight: 50-60 kDa.
2 / 4
IHC testing of FFPE human kidney tissue with NKD2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
3 / 4
Immunofluorescent staining of human A375 cells with NKD2 antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
4 / 4
Flow cytometry testing of human Jurkat cells with NKD2 antibody; Blue=isotype control, Green= NKD2 antibody.

Western blot testing of human Jurkat cell lysate with NKD2 antibody. Expected molecular weight: 50-60 kDa.
IHC testing of FFPE human kidney tissue with NKD2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of human A375 cells with NKD2 antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
Flow cytometry testing of human Jurkat cells with NKD2 antibody; Blue=isotype control, Green= NKD2 antibody.

Further Information

Application Details :
Flow cytometry: 1:25 (1x10e6 cells), Immunofluorescence: 1:25, Immunohistochemistry (FFPE): 1:25, Western blot: 1:500-1:2000
Application Note:
The stated application concentrations are suggested starting points. Titration of the NKD2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Protein naked cuticle homolog 2, also called Naked-2 and NKD2, is a cell autonomous antagonist of the canonical Wnt signaling pathway. [UniProt]
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 366-393 from the human protein was used as the immunogen for the NKD2 antibody.
Limitation:
This NKD2 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
Q969F2