Phospholipase C-L2 Antibody / PLCL2

Contact us
NSJ Bioreagents
Product Code: NSJ-F54629
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54629-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54629-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Phospholipase C-L2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Western blot testing of human NCI-H460 cell lysate with Phospholipase C-L2 antibody. Predicted molecular weight ~126 kDa.
2 / 4
IHC testing of FFPE human brain tissue with Phospholipase C-L2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
3 / 4
Immunofluorescent staining of human NCI-H460 cells with Phospholipase C-L2 antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
4 / 4
Flow cytometry testing of human NCI-H460 cells with Phospholipase C-L2 antibody; Blue=isotype control, Green= Phospholipase C-L2 antibody.
❮❯

Western blot testing of human NCI-H460 cell lysate with Phospholipase C-L2 antibody. Predicted molecular weight ~126 kDa.
IHC testing of FFPE human brain tissue with Phospholipase C-L2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of human NCI-H460 cells with Phospholipase C-L2 antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
Flow cytometry testing of human NCI-H460 cells with Phospholipase C-L2 antibody; Blue=isotype control, Green= Phospholipase C-L2 antibody.

Documents

Further Information

Application Details :
Flow cytometry: 1:25 (1x10e6 cells), Immunofluorescence: 1:25, Immunohistochemistry (FFPE): 1:25, Western blot: 1:500-1:2000
Application Note:
The stated application concentrations are suggested starting points. Titration of the Phospholipase C-L2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
PLCL2 may play an role in the regulation of Ins(1,4,5)P3 around the endoplasmic reticulum (By similarity).
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 1099-1126 from the human protein was used as the immunogen for the Phospholipase C-L2 antibody.
Limitation:
This Phospholipase C-L2 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
Q9UPR0