Protocadherin alpha-C2 Antibody / PCDHAC2

Flow cytometry: 1:25 (1x10e6 cells), Immunofluorescence: 1:25, Immunohistochemistry (FFPE): 1:25, Western blot: 1:500-1:2000

The stated application concentrations are suggested starting points. Titration of the Protocadherin alpha-C2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

This gene is a member of the protocadherin alpha gene cluster, one of three related gene clusters tandemly linked on chromosome five that demonstrate an unusual genomic organization similar to that of B-cell and T-cell receptor gene clusters. The alpha gene cluster is composed of 15 cadherin superfamily genes related to the mouse CNR genes and consists of 13 highly similar and 2 more distantly related coding sequences. The tandem array of 15 N-terminal exons, or variable exons, are followed by downstream C-terminal exons, or constant exons, which are shared by all genes in the cluster. The large, uninterrupted N-terminal exons each encode six cadherin ectodomains while the C-terminal exons encode the cytoplasmic domain. These neural cadherin-like cell adhesion proteins are integral plasma membrane proteins that most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been observed and additional variants have been suggested but their full-length nature has yet to be determined.

Purified

In 1X PBS, pH 7.4, with 0.09% sodium azide

A portion of amino acids 616-644 from the human protein was used as the immunogen for the Protocadherin alpha-C2 antibody.

This Protocadherin alpha-C2 antibody is available for research use only.

Antigen affinity purified

Human

Q9Y5I4