QTRTD1 Antibody / QTRT2

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NSJ Bioreagents
Product Code: NSJ-F54609
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54609-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54609-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the QTRTD1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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IHC testing of FFPE human breast tissue with QTRTD1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 4
Immunofluorescent staining of human MCF7 cells with QTRTD1 antibody (green) and DAPI nuclear stain (blue).
3 / 4
Western blot testing of human MCF7 cell lysate with QTRTD1 antibody. Predicted molecular weight ~47 kDa.
4 / 4
Flow cytometry testing of human MCF7 cells with QTRTD1 antibody; Blue=isotype control, Green= QTRTD1 antibody.
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IHC testing of FFPE human breast tissue with QTRTD1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of human MCF7 cells with QTRTD1 antibody (green) and DAPI nuclear stain (blue).
Western blot testing of human MCF7 cell lysate with QTRTD1 antibody. Predicted molecular weight ~47 kDa.
Flow cytometry testing of human MCF7 cells with QTRTD1 antibody; Blue=isotype control, Green= QTRTD1 antibody.

Documents

Further Information

Application Details :
Flow cytometry: 1:25 (1x10e6 cells), Immunofluorescence: 1:25, Immunohistochemistry (FFPE): 1:25, Western blot: 1:500-1:2000
Application Note:
The stated application concentrations are suggested starting points. Titration of the QTRTD1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Interacts with QTRT1 to form an active queuine tRNA-ribosyltransferase. This enzyme exchanges queuine for the guanine at the wobble position of tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr), thereby forming the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine) (UniProt).
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 321-350 from the human protein was used as the immunogen for the QTRTD1 antibody.
Limitation:
This QTRTD1 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
Q9H974