SCNN1A Antibody

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NSJ Bioreagents
Product Code: NSJ-F54464
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54464-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54464-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the SCNN1A antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 5
IHC testing of FFPE human lung carcinoma tissue with SCNN1A antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 5
Immunofluorescent staining of human HeLa cells with SCNN1A antibody (green) and DAPI nuclear stain (blue).
3 / 5
Western blot testing of human Daudi cell lysate with SCNN1A antibody. Predicted molecular weight ~76 kDa.
4 / 5
Flow cytometry testing of fixed and permeabilized human WiDr cells with SCNN1A antibody; Blue=isotype control, Green= SCNN1A antibody.
5 / 5
Flow cytometry testing of human WiDr cells with SCNN1A antibody; Blue=isotype control, Green= SCNN1A antibody.
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IHC testing of FFPE human lung carcinoma tissue with SCNN1A antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of human HeLa cells with SCNN1A antibody (green) and DAPI nuclear stain (blue).
Western blot testing of human Daudi cell lysate with SCNN1A antibody. Predicted molecular weight ~76 kDa.
Flow cytometry testing of fixed and permeabilized human WiDr cells with SCNN1A antibody; Blue=isotype control, Green= SCNN1A antibody.
Flow cytometry testing of human WiDr cells with SCNN1A antibody; Blue=isotype control, Green= SCNN1A antibody.

Documents

Further Information

Application Details :
Flow cytometry: 1:25 (1x10e6 cells),Immunofluorescence: 1:25,Immunohistochemistry (FFPE): 1:25,Western blot: 1:500-1:2000
Application Note:
The stated application concentrations are suggested starting points. Titration of the SCNN1A antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Nonvoltage-gated, amiloride-sensitive, sodium channels control fluid and electrolyte transport across epithelia in many organs. These channels are heteromeric complexes consisting of 3 subunits: alpha, beta, and gamma. This gene encodes the alpha subunit, and mutations in this gene have been sociated with pseudohypoaldosteronism type 1 (PHA1), a rare salt wasting disease resulting from target organ unresponsiveness to mineralocorticoids.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 365-391 from the human protein was used as the immunogen for the SCNN1A antibody.
Limitation:
This SCNN1A antibody is available for research use only.
Localization:
Cytoplasmic, plasma membrane
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
P37088