TRIM65 Antibody

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NSJ Bioreagents
Product Code: NSJ-F54616
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54616-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54616-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the TRIM65 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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IHC testing of FFPE human prostate carcinoma tissue with TRIM65 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 4
Immunofluorescent staining of human NCI-H460 cells with TRIM65 antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
3 / 4
Western blot testing of human NCI-H460 cell lysate with TRIM65 antibody. Predicted molecular weight ~57 kDa.
4 / 4
Flow cytometry testing of human NCI-H460 cells with TRIM65 antibody; Blue=isotype control, Green= TRIM65 antibody.
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IHC testing of FFPE human prostate carcinoma tissue with TRIM65 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of human NCI-H460 cells with TRIM65 antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
Western blot testing of human NCI-H460 cell lysate with TRIM65 antibody. Predicted molecular weight ~57 kDa.
Flow cytometry testing of human NCI-H460 cells with TRIM65 antibody; Blue=isotype control, Green= TRIM65 antibody.

Documents

Further Information

Application Details :
Flow cytometry: 1:25 (1x10e6 cells), Immunofluorescence: 1:25, Immunohistochemistry (FFPE): 1:25, Western blot: 1:500-1:2000
Application Note:
The stated application concentrations are suggested starting points. Titration of the TRIM65 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
TRIM65 belongs to the TRIM/RBCC family. It contains one B box-type zinc finger, one B30.2/SPRY domain and one RING-type zinc finger.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 327-355 from the human protein was used as the immunogen for the TRIM65 antibody.
Limitation:
This TRIM65 antibody is available for research use only.
Localization:
Cytoplasmic, nuclear
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
Q6PJ69