RFC5 Antibody

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NSJ Bioreagents
Product Code: NSJ-F54778
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54778-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54778-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
Aliquot the RFC5 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with RFC5 antibody.
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Western blot testing of human HeLa cell lysate with RFC5 antibody. Predicted molecular weight ~38 kDa.
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Flow cytometry testing of human HeLa cells with RFC5 antibody; Blue=isotype control, Green= RFC5 antibody.
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Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with RFC5 antibody.
Western blot testing of human HeLa cell lysate with RFC5 antibody. Predicted molecular weight ~38 kDa.
Flow cytometry testing of human HeLa cells with RFC5 antibody; Blue=isotype control, Green= RFC5 antibody.

Documents

Further Information

Application Details :
Flow cytometry: 1:25 (1x10e6 cells), Western blot: 1:500-1:2000
Application Note:
The stated application concentrations are suggested starting points. Titration of the RFC5 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. RFC5 is the 36 kD subunit. This subunit can interact with the C-terminal region of PCNA. It forms a core complex with the 38 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 170-200 from the human protein was used as the immunogen for the RFC5 antibody.
Limitation:
This RFC5 antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human
Uniprot #:
P40937