EEF1A1/2 Antibody

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NSJ Bioreagents
Product Code: NSJ-F54864
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54864-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54864-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the EEF1A1/2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot testing of human 293T cell lysate with EEF1A1/2 antibody and a GADPH loading control Ab. Predicted molecular weight ~50 kDa.
2 / 4
Western blot testing of human 1) Y79 and 2) T-47D cell lysate with EEF1A1/2 antibody. Predicted molecular weight ~50 kDa.
3 / 4
IHC testing of FFPE human brain tissue with EEF1A1/2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
4 / 4
Flow cytometry testing of human NCI-H292 cells with EEF1A1/2 antibody; Blue=isotype control, Green= EEF1A1/2 antibody.
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Western blot testing of human 293T cell lysate with EEF1A1/2 antibody and a GADPH loading control Ab. Predicted molecular weight ~50 kDa.
Western blot testing of human 1) Y79 and 2) T-47D cell lysate with EEF1A1/2 antibody. Predicted molecular weight ~50 kDa.
IHC testing of FFPE human brain tissue with EEF1A1/2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of human NCI-H292 cells with EEF1A1/2 antibody; Blue=isotype control, Green= EEF1A1/2 antibody.

Documents

Further Information

Application Details :
Flow cytometry: 1:10-1:50 (1x10e6 cells), Western blot: 1:500-1:1000, Immunohistochemistry (FFPE): 1:50-1:100
Application Note:
The stated application concentrations are suggested starting points. Titration of the EEF1A1/2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
EEF1A1 is an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. This isoform is identified as an autoantigen in 66% of patients with Felty syndrome.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 46-72 from the human EEF1A1 protein was used as the immunogen for the EEF1A1/2 antibody.
Limitation:
This EEF1A1/2 antibody is available for research use only.
Localization:
Cytoplasmic, nuclear
Purity:
Purified
Species Reactivity :
Human
Uniprot #:
P68104