Autotaxin Antibody / ENPP2

NSJ Bioreagents
Product Code: NSJ-F54782
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54782-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54782-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the ENPP2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Western blot testing of mouse cerebellum tissue lysate with ENPP2 antibody. Expected molecular weight: 99-125 kDa depending on glycosylation level.
2 / 4
Western blot testing of human Y79 cell lysate with ENPP2 antibody. Expected molecular weight: 99-125 kDa depending on glycosylation level.
3 / 4
IHC testing of FFPE human hepatocellular carcinoma tissue with ENPP2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
4 / 4
Flow cytometry testing of human MDA-MB-435 cells with ENPP2 antibody; Blue=isotype control, Green= ENPP2 antibody.

Western blot testing of mouse cerebellum tissue lysate with ENPP2 antibody. Expected molecular weight: 99-125 kDa depending on glycosylation level.
Western blot testing of human Y79 cell lysate with ENPP2 antibody. Expected molecular weight: 99-125 kDa depending on glycosylation level.
IHC testing of FFPE human hepatocellular carcinoma tissue with ENPP2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of human MDA-MB-435 cells with ENPP2 antibody; Blue=isotype control, Green= ENPP2 antibody.

Further Information

Application Details :
Flow cytometry: 1:25 (1x10e6 cells), Western blot: 1:500-1:1000, Immunohistochemistry (FFPE): 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the ENPP2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
ENPP2 functions as both a phosphodiesterase, which cleaves phosphodiester bonds at the 5' end of oligonucleotides, and a phospholipase, which catalyzes production of lysophosphatidic acid (LPA) in extracellular fluids. LPA evokes growth factor-like responses including stimulation of cell proliferation and chemotaxis. This protein stimulates the motility of tumor cells and has angiogenic properties, and its expression is upregulated in several kinds of carcinomas. This protein is secreted and further processed to make the biologically active form.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 401-430 from the human protein was used as the immunogen for the ENPP2 antibody.
Limitation:
This ENPP2 antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human
Uniprot #:
Q13822