GZMB Antibody / Granzyme B

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NSJ Bioreagents
Product Code: NSJ-F54859
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54859-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54859-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the GZMB antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Flow cytometry testing of human HL60 cells with GZMB antibody; Blue=isotype control, Green= GZMB antibody.
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Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with GZMB antibody.
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Western blot testing of human HL60 cell lysate with GZMB antibody. Predicted molecular weight: 29-37 kDa.
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IHC testing of FFPE human lymph tissue with GZMB antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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Flow cytometry testing of human HL60 cells with GZMB antibody; Blue=isotype control, Green= GZMB antibody.
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with GZMB antibody.
Western blot testing of human HL60 cell lysate with GZMB antibody. Predicted molecular weight: 29-37 kDa.
IHC testing of FFPE human lymph tissue with GZMB antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Documents

Further Information

Application Details :
Flow cytometry: 1:10-1:50 (1x10e6 cells), Western blot: 1:500-1:1000, Immunohistochemistry (FFPE): 1:50-1:100
Application Note:
The stated application concentrations are suggested starting points. Titration of the GZMB antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein is crucial for the rapid induction of target cell apoptosis by CTL in cell-mediated immune response.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 175-203 from the human protein was used as the immunogen for the GZMB antibody.
Limitation:
This GZMB antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Purified
Species Reactivity :
Human
Uniprot #:
P10144