TAGLN Antibody / Transgelin / SM22 alpha

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NSJ Bioreagents
Product Code: NSJ-F54870
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54870-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54870-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the TAGLN antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot testing of human SK-BR-3 cell lysate with TAGLN antibody. Predicted molecular weight ~22 kDa.
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Western blot testing of human uterus tissue lysate with TAGLN antibody. Predicted molecular weight ~22 kDa.
3 / 4
IHC testing of FFPE human skeletal muscle tissue with TAGLN antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
4 / 4
Flow cytometry testing of human MDA-MB-468 cells with TAGLN antibody; Blue=isotype control, Green= TAGLN antibody.
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Western blot testing of human SK-BR-3 cell lysate with TAGLN antibody. Predicted molecular weight ~22 kDa.
Western blot testing of human uterus tissue lysate with TAGLN antibody. Predicted molecular weight ~22 kDa.
IHC testing of FFPE human skeletal muscle tissue with TAGLN antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of human MDA-MB-468 cells with TAGLN antibody; Blue=isotype control, Green= TAGLN antibody.

Documents

Further Information

Application Details :
Flow cytometry: 1:10-1:50 (1x10e6 cells), Western blot: 1:500-1:1000, Immunohistochemistry (FFPE): 1:50-1:100
Application Note:
The stated application concentrations are suggested starting points. Titration of the TAGLN antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
TAGLN is a transformation and shape-change sensitive actin cross-linking/gelling protein found in fibroblasts and smooth muscle. Its expression is down-regulated in many cell lines, and this down-regulation may be an early and sensitive marker for the onset of transformation. A functional role of this protein is unclear.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 24-51 from the human protein was used as the immunogen for the TAGLN antibody.
Limitation:
This TAGLN antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Purified
Species Reactivity :
Human
Uniprot #:
Q01995