TPM4 Antibody / Tropomyosin 4

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NSJ Bioreagents
Product Code: NSJ-F54657
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54657-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54657-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Tropomyosin 4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
IHC testing of FFPE human skeletal muscle tissue with Tropomyosin 4 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 4
IHC testing of FFPE human breast tissue with Tropomyosin 4 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
3 / 4
Western blot testing of human 1) U-251, 2) A549 and 3) Jurkat cell lysate with Tropomyosin 4 antibody. Predicted molecular weight ~33 kDa.
4 / 4
Flow cytometry testing of human U-251 cells with Tropomyosin 4 antibody; Left=isotype control, Right= Tropomyosin 4 antibody.
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IHC testing of FFPE human skeletal muscle tissue with Tropomyosin 4 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE human breast tissue with Tropomyosin 4 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of human 1) U-251, 2) A549 and 3) Jurkat cell lysate with Tropomyosin 4 antibody. Predicted molecular weight ~33 kDa.
Flow cytometry testing of human U-251 cells with Tropomyosin 4 antibody; Left=isotype control, Right= Tropomyosin 4 antibody.

Documents

Further Information

Application Details :
Flow cytometry: 1:25 (1x10e6 cells), Western blot: 1:500-1:2000, Immunohistochemistry (FFPE): 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the Tropomyosin 4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
The TPM4 gene encodes a member of the tropomyosin family of actin-binding proteins involved in the contractile system of striated and smooth muscles and the cytoskeleton of non-muscle cells. Tropomyosins are dimers of coiled-coil proteins that polymerize end-to-end along the major groove in most actin filaments. They provide stability to the filaments and regulate access of other actin-binding proteins. In muscle cells, they regulate muscle contraction by controlling the binding of myosin heads to the actin filament. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 26-54 from the human protein was used as the immunogen for the Tropomyosin 4 antibody.
Limitation:
This Tropomyosin 4 antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
P67936