ARHGDIA Antibody / RHOGDI Antibody

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NSJ Bioreagents
Product Code: NSJ-F54788
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54788-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54788-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the ARHGDIA antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
IHC testing of FFPE human brain tissue with ARHGDIA antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 4
Western blot testing of human 1) A375, 2) HL60 and 3) Ramos cell lysate with ARHGDIA antibody. Expected molecular weight: 23-29 kDa.
3 / 4
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with ARHGDIA antibody.
4 / 4
Flow cytometry testing of human HL60 cells with ARHGDIA antibody; Blue=isotype control, Green= ARHGDIA antibody.
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IHC testing of FFPE human brain tissue with ARHGDIA antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of human 1) A375, 2) HL60 and 3) Ramos cell lysate with ARHGDIA antibody. Expected molecular weight: 23-29 kDa.
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with ARHGDIA antibody.
Flow cytometry testing of human HL60 cells with ARHGDIA antibody; Blue=isotype control, Green= ARHGDIA antibody.

Documents

Further Information

Application Details :
Flow cytometry: 1:25 (1x10e6 cells), Immunohistochemistry (FFPE): 1:25, Western blot: 1:500-1:1000
Application Note:
The stated application concentrations are suggested starting points. Titration of the ARHGDIA antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
ARHGDIA regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 112-140 from the human protein was used as the immunogen for the ARHGDIA antibody.
Limitation:
This ARHGDIA antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Purified
Species Reactivity :
Human
Uniprot #:
P52565