BGN Antibody / Biglycan

NSJ Bioreagents
Product Code: NSJ-F54881
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54881-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54881-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the BGN antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot testing of human SK-BR-3 cell lysate with BGN antibody. Predicted molecular weight ~41 kDa.
2 / 4
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with BGN antibody.
3 / 4
IHC testing of FFPE human brain tissue with BGN antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
4 / 4
Flow cytometry testing of human A375 cells with BGN antibody; Blue=isotype control, Green= BGN antibody.

Western blot testing of human SK-BR-3 cell lysate with BGN antibody. Predicted molecular weight ~41 kDa.
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with BGN antibody.
IHC testing of FFPE human brain tissue with BGN antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of human A375 cells with BGN antibody; Blue=isotype control, Green= BGN antibody.

Further Information

Application Details :
Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunohistochemistry (FFPE): 1:50-1:100, Western blot: 1:500-1:1000
Application Note:
The stated application concentrations are suggested starting points. Titration of the BGN antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
BGN is a small cellular or pericellular matrix proteoglycan that is closely related in structure to two other small proteoglycans, decorin and fibromodulin. The protein and decorin are thought to be the result of a gene duplication. Decorin contains one attached glycosaminoglycan chain, while this protein probably contains two chains. For this reason, this protein is called biglycan. This protein is thought to function in connective tissue metabolism by binding to collagen fibrils and transfering growth factor-beta. It may promote neuronal survival.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 148-176 from the human protein was used as the immunogen for the BGN antibody.
Limitation:
This BGN antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human
Uniprot #:
P21810