Claudin 2 Antibody / CLDN2

NSJ Bioreagents
Product Code: NSJ-F54654
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54654-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54654-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the CLDN2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Western blot testing of human heart tissue lysate with CLDN2 antibody. Predicted molecular weight ~25 kDa.
2 / 4
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with CLDN2 antibody. Predicted molecular weight ~25 kDa.
3 / 4
IHC testing of FFPE human stomach tissue with CLDN2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
4 / 4
Flow cytometry testing of human HEK293 cells with CLDN2 antibody; Left=isotype control, Right= CLDN2 antibody.

Western blot testing of human heart tissue lysate with CLDN2 antibody. Predicted molecular weight ~25 kDa.
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with CLDN2 antibody. Predicted molecular weight ~25 kDa.
IHC testing of FFPE human stomach tissue with CLDN2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of human HEK293 cells with CLDN2 antibody; Left=isotype control, Right= CLDN2 antibody.

Further Information

Application Details :
Flow cytometry: 1:25 (1x10e6 cells), Immunohistochemistry (FFPE): 1:25, Western blot: 1:500-1:2000
Application Note:
The stated application concentrations are suggested starting points. Titration of the CLDN2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This gene product belongs to the claudin protein family whose members have been identified as major integral membrane proteins localized exclusively at tight junctions. Claudins are expressed in an organ-specific manner and regulate tissue-specific physiologic properties of tight junctions. This protein is expressed in the intestine. Alternatively spliced transcript variants with different 5' untranslated region have been found for this gene.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 200-227 from the human protein was used as the immunogen for the CLDN2 antibody.
Limitation:
This CLDN2 antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
P57739