DENND1B Antibody

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NSJ Bioreagents
Product Code: NSJ-F54541
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54541-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54541-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the DENND1B antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot testing of human NCI-H460 cell lysate with DENND1B antibody. Predicted molecular weight: 34~87 kDa (multiple isoforms).
2 / 4
Western blot testing of human MOLT4 cell lysate with DENND1B antibody. Predicted molecular weight: 34~87 kDa (multiple isoforms).
3 / 4
IHC testing of FFPE human lymph tissue with DENND1B antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
4 / 4
Flow cytometry testing of human NCI-H460 cells with DENND1B antibody; Blue=isotype control, Green= DENND1B antibody.
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Western blot testing of human NCI-H460 cell lysate with DENND1B antibody. Predicted molecular weight: 34~87 kDa (multiple isoforms).
Western blot testing of human MOLT4 cell lysate with DENND1B antibody. Predicted molecular weight: 34~87 kDa (multiple isoforms).
IHC testing of FFPE human lymph tissue with DENND1B antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of human NCI-H460 cells with DENND1B antibody; Blue=isotype control, Green= DENND1B antibody.

Documents

Further Information

Application Details :
Flow cytometry: 1:25 (1x10e6 cells), Immunohistochemistry (FFPE): 1:25, Western blot: 1:500-1:2000
Application Note:
The stated application concentrations are suggested starting points. Titration of the DENND1B antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Clathrin-mediated endocytosis is a major mechanism for internalization of proteins and lipids. Members of the connecdenn family, such as DENND1B, function as guanine nucleotide exchange factors (GEFs) for the early endosomal small GTPase RAB35 and bind to clathrin and clathrin adaptor protein-2. Thus, connecdenns link RAB35 activation with the clathrin machinery.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 39-68 from the human protein was used as the immunogen for the DENND1B antibody.
Limitation:
This DENND1B antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
Q6P3S1