ERP29 Antibody

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NSJ Bioreagents
Product Code: NSJ-F54790
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54790-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54790-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Endoplasmic reticulum resident protein 29 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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IHC testing of FFPE human hepatocellular carcinoma tissue with Endoplasmic reticulum resident protein 29 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 4
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with Endoplasmic reticulum resident protein 29 antibody.
3 / 4
Western blot testing of human 1) A2058, 2) A375, 3) MCF7, 4) NCI-H460 and 5) Y79 cell lysate with Endoplasmic reticulum resident protein 29 antibody. Predicted molecular weight: ~29 kDa.
4 / 4
Flow cytometry testing of human NCI-H292 cells with Endoplasmic reticulum resident protein 29 antibody; Blue=isotype control, Green= Endoplasmic reticulum resident protein 29 antibody.
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IHC testing of FFPE human hepatocellular carcinoma tissue with Endoplasmic reticulum resident protein 29 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with Endoplasmic reticulum resident protein 29 antibody.
Western blot testing of human 1) A2058, 2) A375, 3) MCF7, 4) NCI-H460 and 5) Y79 cell lysate with Endoplasmic reticulum resident protein 29 antibody. Predicted molecular weight: ~29 kDa.
Flow cytometry testing of human NCI-H292 cells with Endoplasmic reticulum resident protein 29 antibody; Blue=isotype control, Green= Endoplasmic reticulum resident protein 29 antibody.

Documents

Further Information

Application Details :
Flow cytometry: 1:25 (1x10e6 cells), Immunohistochemistry (FFPE): 1:25, Western blot: 1:500-1:1000
Application Note:
The stated application concentrations are suggested starting points. Titration of the Endoplasmic reticulum resident protein 29 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
ERP29 shows sequence similarity to the protein disulfide isomerase family. However, it lacks the thioredoxin motif characteristic of this family, suggesting that this protein does not function as a disulfide isomerase. The protein dimerizes and is thought to play a role in the processing of secretory proteins within the ER.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 151-179 from the human protein was used as the immunogen for the Endoplasmic reticulum resident protein 29 antibody.
Limitation:
This Endoplasmic reticulum resident protein 29 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Purified
Species Reactivity :
Human
Uniprot #:
P30040