FBX24 Antibody / FBXO24

NSJ Bioreagents
Product Code: NSJ-F54569
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54569-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54569-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the FBX24 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 3
IHC testing of FFPE human testis tissue with FBX24 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 3
Western blot testing of human A549 cell lysate with FBX24 antibody. Predicted molecular weight: 36-69 kDa (multiple isoforms).
3 / 3
Flow cytometry testing of human A549 cells with FBX24 antibody; Blue=isotype control, Green= FBX24 antibody.

IHC testing of FFPE human testis tissue with FBX24 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of human A549 cell lysate with FBX24 antibody. Predicted molecular weight: 36-69 kDa (multiple isoforms).
Flow cytometry testing of human A549 cells with FBX24 antibody; Blue=isotype control, Green= FBX24 antibody.

Further Information

Application Details :
Flow cytometry: 1:25 (1x10e6 cells), Immunohistochemistry (FFPE): 1:25, Western blot: 1:500-1:2000
Application Note:
The stated application concentrations are suggested starting points. Titration of the FBX24 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 118-145 from the human protein was used as the immunogen for the FBX24 antibody.
Limitation:
This FBX24 antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
O75426