Ferritin heavy chain Antibody / FTH1

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NSJ Bioreagents
Product Code: NSJ-F54896
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54896-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54896-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Ferritin heavy chain antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
Western blot testing of human 1) HeLa and 2) HT-29 cell lysate with Ferritin heavy chain antibody. Predicted molecular weight ~21 kDa.
2 / 4
Western blot testing of human A2058 cell lysate with Ferritin heavy chain antibody. Predicted molecular weight ~21 kDa.
3 / 4
IHC testing of FFPE human hepatocellular carcinoma tissue with Ferritin heavy chain antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
4 / 4
Flow cytometry testing of human HepG2 cells with Ferritin heavy chain antibody; Blue=isotype control, Green= Ferritin heavy chain antibody.
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Western blot testing of human 1) HeLa and 2) HT-29 cell lysate with Ferritin heavy chain antibody. Predicted molecular weight ~21 kDa.
Western blot testing of human A2058 cell lysate with Ferritin heavy chain antibody. Predicted molecular weight ~21 kDa.
IHC testing of FFPE human hepatocellular carcinoma tissue with Ferritin heavy chain antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of human HepG2 cells with Ferritin heavy chain antibody; Blue=isotype control, Green= Ferritin heavy chain antibody.

Documents

Further Information

Application Details :
Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunohistochemistry (FFPE): 1:50-1:100, Western blot: 1:500-1:1000
Application Note:
The stated application concentrations are suggested starting points. Titration of the Ferritin heavy chain antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
FTH1 is the heavy subunit of ferritin, the major intracellular iron storage protein in prokaryotes and eukaryotes. It is composed of 24 subunits of the heavy and light ferritin chains. Variation in ferritin subunit composition may affect the rates of iron uptake and release in different tissues. A major function of ferritin is the storage of iron in a soluble and nontoxic state. Defects in ferritin proteins are associated with several neurodegenerative diseases.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 154-183 from the human protein was used as the immunogen for the Ferritin heavy chain antibody.
Limitation:
This Ferritin heavy chain antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
P02794