FGFBP3 Antibody / Fibroblast growth factor-binding protein 3

NSJ Bioreagents
Product Code: NSJ-F54831
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54831-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54831-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the FGFBP3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 3
IHC testing of FFPE human brain tissue with FGFBP3 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 3
Western blot testing of human 1) brain and 2) HL60 cell lysate with FGFBP3 antibody. Predicted molecular weight ~28 kDa.
3 / 3
Flow cytometry testing of human HEK293 cells with FGFBP3 antibody; Blue=isotype control, Green= FGFBP3 antibody.

IHC testing of FFPE human brain tissue with FGFBP3 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of human 1) brain and 2) HL60 cell lysate with FGFBP3 antibody. Predicted molecular weight ~28 kDa.
Flow cytometry testing of human HEK293 cells with FGFBP3 antibody; Blue=isotype control, Green= FGFBP3 antibody.

Further Information

Application Details :
Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunohistochemistry (FFPE): 1:50-1:100, Western blot: 1:500-1:1000
Application Note:
The stated application concentrations are suggested starting points. Titration of the FGFBP3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Heparin-binding protein which binds to FGF2, prevents binding of FGF2 to heparin and probably inhibits immobilization of FGF2 on extracellular matrix glycosaminoglycans, allowing its release and subsequent activation of FGFR signaling which leads to increased vascular permeability. [UniProt]
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 181-210 from the human protein was used as the immunogen for the FGFBP3 antibody.
Limitation:
This FGFBP3 antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
Q8TAT2