FHR-5 Antibody / Complement factor H-related protein 5 / CFHR5

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NSJ Bioreagents
Product Code: NSJ-F54834
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54834-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54834-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the FHR-5 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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IHC testing of FFPE human hepatocellular carcinoma tissue with FHR-5 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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IHC testing of FFPE human liver tissue with FHR-5 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
3 / 4
Western blot testing of human 1) CCRF-CEM and 2) K562 cell lysate with FHR-5 antibody. Predicted molecular weight ~64 kDa.
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Flow cytometry testing of human CCRF-CEM cells with FHR-5 antibody; Blue=isotype control, Green= FHR-5 antibody.
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IHC testing of FFPE human hepatocellular carcinoma tissue with FHR-5 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE human liver tissue with FHR-5 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of human 1) CCRF-CEM and 2) K562 cell lysate with FHR-5 antibody. Predicted molecular weight ~64 kDa.
Flow cytometry testing of human CCRF-CEM cells with FHR-5 antibody; Blue=isotype control, Green= FHR-5 antibody.

Documents

Further Information

Application Details :
Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunohistochemistry (FFPE): 1:5-1:100, Western blot: 1:500-1:1000
Application Note:
The stated application concentrations are suggested starting points. Titration of the FHR-5 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
CFHR5 / FHR-5 is a member of a small complement factor H (CFH) gene cluster on chromosome 1. Each member of this gene family contains multiple short consensus repeats (SCRs) typical of regulators of complement activation. The protein encoded by this gene has nine SCRs with the first two repeats having heparin binding properties, a region within repeats 5-7 having heparin binding and C reactive protein binding properties, and the C-terminal repeats being similar to a complement component 3 b (C3b) binding domain. This protein co-localizes with C3, binds C3b in a dose-dependent manner, and is recruited to tissues damaged by C-reactive protein. Allelic variations in this gene have been associated, but not causally linked, with two different forms of kidney disease: membranoproliferative glomerulonephritis type II (MPGNII) and hemolytic uraemic syndrome (HUS). [provided by RefSeq].
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 203-231 from the human protein was used as the immunogen for the FHR-5 antibody.
Limitation:
This FHR-5 antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
Q9BXR6