Galactose-1-phosphate uridylyltransferase Antibody / GALT

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NSJ Bioreagents
Product Code: NSJ-F54894
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54894-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54894-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Galactose-1-phosphate uridylyltransferase antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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IHC testing of FFPE human breast carcinoma tissue with Galactose-1-phosphate uridylyltransferase antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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Flow cytometry testing of human MDA-MB-468 cells with Galactose-1-phosphate uridylyltransferase antibody; Blue=isotype control, Green= Galactose-1-phosphate uridylyltransferase antibody.
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Western blot testing of human HepG2 cell lysate with Galactose-1-phosphate uridylyltransferase antibody. Predicted molecular weight ~43 kDa.
4 / 4
Western blot testing of human MDA-MB-231 cell lysate with Galactose-1-phosphate uridylyltransferase antibody. Predicted molecular weight ~43 kDa.
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IHC testing of FFPE human breast carcinoma tissue with Galactose-1-phosphate uridylyltransferase antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of human MDA-MB-468 cells with Galactose-1-phosphate uridylyltransferase antibody; Blue=isotype control, Green= Galactose-1-phosphate uridylyltransferase antibody.
Western blot testing of human HepG2 cell lysate with Galactose-1-phosphate uridylyltransferase antibody. Predicted molecular weight ~43 kDa.
Western blot testing of human MDA-MB-231 cell lysate with Galactose-1-phosphate uridylyltransferase antibody. Predicted molecular weight ~43 kDa.

Documents

Further Information

Application Details :
Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunohistochemistry (FFPE): 1:50-1:100, Western blot: 1:500-1:1000
Application Note:
The stated application concentrations are suggested starting points. Titration of the Galactose-1-phosphate uridylyltransferase antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Galactose-1-phosphate uridyl transferase (GALT) catalyzes the second step of the Leloir pathway of galactose metabolism, namely the conversion of UDP-glucose + galactose-1-phosphate to glucose-1-phosphate + UDP-galactose. The absence of this enzyme results in classic galactosemia in humans and can be fatal in the newborn period if lactose is not removed from the diet.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 344-373 from the human protein was used as the immunogen for the Galactose-1-phosphate uridylyltransferase antibody.
Limitation:
This Galactose-1-phosphate uridylyltransferase antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
P07902