HNRPL Antibody / hnRNP L

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NSJ Bioreagents
Product Code: NSJ-F54803
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54803-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54803-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the HNRPL antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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IHC testing of FFPE human hepatocellular carcinoma tissue with HNRPL antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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Western blot testing of human 1) HepG2 and 2) Jurkat cell lysate with HNRPL antibody. Predicted molecular weight ~64 kDa.
3 / 4
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with HNRPL antibody.
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Flow cytometry testing of human Jurkat cells with HNRPL antibody; Blue=isotype control, Green= HNRPL antibody.
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IHC testing of FFPE human hepatocellular carcinoma tissue with HNRPL antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of human 1) HepG2 and 2) Jurkat cell lysate with HNRPL antibody. Predicted molecular weight ~64 kDa.
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with HNRPL antibody.
Flow cytometry testing of human Jurkat cells with HNRPL antibody; Blue=isotype control, Green= HNRPL antibody.

Documents

Further Information

Application Details :
Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunohistochemistry (FFPE): 1:50-1:100, Western blot: 1:500-1:1000
Application Note:
The stated application concentrations are suggested starting points. Titration of the HNRPL antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
HNRPL is a component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. L is associated with most nascent transcripts including those of the landmark giant loops of amphibian lampbrush chromosomes.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 249-277 from the human protein was used as the immunogen for the HNRPL antibody.
Limitation:
This HNRPL antibody is available for research use only.
Localization:
Cytoplasmic, nuclear
Purity:
Purified
Species Reactivity :
Human
Uniprot #:
P14866