JHDM1D Antibody / KDM7A

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NSJ Bioreagents
Product Code: NSJ-F54415
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54415-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54415-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the JHDM1D antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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IHC testing of FFPE human testis tissue with JHDM1D antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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Western blot testing of human HEK293 cell lysate with JHDM1D antibody.
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Flow cytometry testing of fixed and permeabilized human HEK293 cells with JHDM1D antibody; Blue=isotype control, Green= JHDM1D antibody.
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IHC testing of FFPE human testis tissue with JHDM1D antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of human HEK293 cell lysate with JHDM1D antibody.
Flow cytometry testing of fixed and permeabilized human HEK293 cells with JHDM1D antibody; Blue=isotype control, Green= JHDM1D antibody.

Documents

Further Information

Application Details :
Flow cytometry: 1:25 (1x10e6 cells),Immunohistochemistry (FFPE): 1:25,Western blot: 1:500-1:2000
Application Note:
The stated application concentrations are suggested starting points. Titration of the JHDM1D antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
JHDM1D histone demethylase that specifically demethylates dimethylated 'Lys-9' and 'Lys-27' (H3K9me2 and H3K27me2, respectively) of histone H3, thereby playing a central role in histone code. This specifically binds trimethylated 'Lys-4' of histone H3 (H3K4me3), affecting histone demethylase specificity: in presence of H3K4me3, it has no demethylase activity toward H3K9me2, while it has high activity toward H3K27me2. This demethylates H3K9me2 in absence of H3K4me3.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 290-316 from the human protein was used as the immunogen for the JHDM1D antibody.
Limitation:
This JHDM1D antibody is available for research use only.
Localization:
Nuclear
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
Q6ZMT4