Lymphocyte Specific Protein 1 Antibody / LSP1

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NSJ Bioreagents
Product Code: NSJ-F54824
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54824-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54824-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Lymphocyte Specific Protein 1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot testing of human 1) Daudi and 2) Ramos cell lysate with Lymphocyte Specific Protein 1 antibody. Predicted molecular weight: ~37 kDa but is routinely observed at ~52 kDa (PMID: 15684321).
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Western blot testing of human MCF7 cell lysate with Lymphocyte Specific Protein 1 antibody. Predicted molecular weight: ~37 kDa but is routinely observed at ~52 kDa (PMID: 15684321).
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IHC testing of FFPE human lymph node tissue with Lymphocyte Specific Protein 1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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Flow cytometry testing of human MCF7 cells with Lymphocyte Specific Protein 1 antibody; Blue=isotype control, Green= Lymphocyte Specific Protein 1 antibody.
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Western blot testing of human 1) Daudi and 2) Ramos cell lysate with Lymphocyte Specific Protein 1 antibody. Predicted molecular weight: ~37 kDa but is routinely observed at ~52 kDa (PMID: 15684321).
Western blot testing of human MCF7 cell lysate with Lymphocyte Specific Protein 1 antibody. Predicted molecular weight: ~37 kDa but is routinely observed at ~52 kDa (PMID: 15684321).
IHC testing of FFPE human lymph node tissue with Lymphocyte Specific Protein 1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of human MCF7 cells with Lymphocyte Specific Protein 1 antibody; Blue=isotype control, Green= Lymphocyte Specific Protein 1 antibody.

Documents

Further Information

Application Details :
Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunohistochemistry (FFPE): 1:50-1:100, Western blot: 1:2000-1:8000
Application Note:
The stated application concentrations are suggested starting points. Titration of the Lymphocyte Specific Protein 1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
LSP1 / Lymphocyte Specific Protein 1 is an intracellular F-actin binding protein. The protein is expressed in lymphocytes, neutrophils, macrophages, and endothelium and may regulate neutrophil motility, adhesion to fibrinogen matrix proteins, and transendothelial migration. Alternative splicing results in multiple transcript variants encoding different isoforms.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 108-134 from the human protein was used as the immunogen for the Lymphocyte Specific Protein 1 antibody.
Limitation:
This Lymphocyte Specific Protein 1 antibody is available for research use only.
Localization:
Cytoplasmic, membrane
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
P33241