Mitoferrin-2 Antibody / MFRN2 / SLC25A28

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NSJ Bioreagents
Product Code: NSJ-F54817
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54817-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54817-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Mitoferrin-2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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Western blot testing of human Jurkat cell lysate with Mitoferrin-2 antibody. Predicted molecular weight ~39 kDa.
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Western blot testing of human MCF7 cell lysate with Mitoferrin-2 antibody. Predicted molecular weight ~39 kDa.
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IHC testing of FFPE human lung carcinoma tissue with Mitoferrin-2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
4 / 4
Flow cytometry testing of human Jurkat cells with Mitoferrin-2 antibody; Blue=isotype control, Green= Mitoferrin-2 antibody.
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Western blot testing of human Jurkat cell lysate with Mitoferrin-2 antibody. Predicted molecular weight ~39 kDa.
Western blot testing of human MCF7 cell lysate with Mitoferrin-2 antibody. Predicted molecular weight ~39 kDa.
IHC testing of FFPE human lung carcinoma tissue with Mitoferrin-2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of human Jurkat cells with Mitoferrin-2 antibody; Blue=isotype control, Green= Mitoferrin-2 antibody.

Documents

Further Information

Application Details :
Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunohistochemistry (FFPE): 1:50-1:100, Western blot: 1:500-1:1000
Application Note:
The stated application concentrations are suggested starting points. Titration of the Mitoferrin-2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
MFRN2 is mitochondrial iron transporter that mediates iron uptake. It is probably required for heme synthesis of hemoproteins and Fe-S cluster assembly in non-erythroid cells. The iron delivered into the mitochondria, presumably as Fe(2+), is then probably delivered to ferrochelatase to catalyze Fe(2+) incorporation into protoprophyrin IX to make heme.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 36-64 from the human protein was used as the immunogen for the Mitoferrin-2 antibody.
Limitation:
This Mitoferrin-2 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
Q96A46