PGD Antibody / 6-Phosphogluconate Dehydrogenase

NSJ Bioreagents
Product Code: NSJ-F54826
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54826-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54826-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the PGD antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 3
IHC testing of FFPE human lung carcinoma tissue with PGD antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 3
Western blot testing of human 1) HEK293 and 2) MCF7 cell lysate with PGD antibody. Predicted molecular weight ~53 kDa.
3 / 3
Flow cytometry testing of human HEK293 cells with PGD antibody; Blue=isotype control, Green= PGD antibody.

IHC testing of FFPE human lung carcinoma tissue with PGD antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of human 1) HEK293 and 2) MCF7 cell lysate with PGD antibody. Predicted molecular weight ~53 kDa.
Flow cytometry testing of human HEK293 cells with PGD antibody; Blue=isotype control, Green= PGD antibody.

Further Information

Application Details :
Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunohistochemistry (FFPE): 1:50-1:100, Western blot: 1:500-1:1000
Application Note:
The stated application concentrations are suggested starting points. Titration of the PGD antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
6-phosphogluconate dehydrogenase is the second dehydrogenase in the pentose phosphate shunt. Deficiency of this enzyme is generally asymptomatic, and the inheritance of this disorder is autosomal dominant. Hemolysis results from combined deficiency of 6-phosphogluconate dehydrogenase and 6-phosphogluconolactonase suggesting a synergism of the two enzymopathies.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 236-265 from the human protein was used as the immunogen for the PGD antibody.
Limitation:
This PGD antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
P52209