Peroxiredoxin 6 Antibody / PRDX6 / aiPLA2

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NSJ Bioreagents
Product Code: NSJ-F54799
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54799-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54799-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the PRDX6 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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IHC testing of FFPE human brain tissue with PRDX6 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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Western blot testing of human CCRF-CEM cell lysate with PRDX6 antibody. Predicted molecular weight ~25 kDa.
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Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with PRDX6 antibody.
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Flow cytometry testing of human CCRF-CEM cells with PRDX6 antibody; Blue=isotype control, Green= PRDX6 antibody.
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IHC testing of FFPE human brain tissue with PRDX6 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of human CCRF-CEM cell lysate with PRDX6 antibody. Predicted molecular weight ~25 kDa.
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with PRDX6 antibody.
Flow cytometry testing of human CCRF-CEM cells with PRDX6 antibody; Blue=isotype control, Green= PRDX6 antibody.

Documents

Further Information

Application Details :
Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunohistochemistry (FFPE): 1:50-1:100, Western blot: 1:500-1:1000
Application Note:
The stated application concentrations are suggested starting points. Titration of the PRDX6 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
PRDX6 is a member of the thiol-specific antioxidant protein family. This protein is a bifunctional enzyme with two distinct active sites. It is involved in redox regulation of the cell; it can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. It may play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 197-224 from the human protein was used as the immunogen for the PRDX6 antibody.
Limitation:
This PRDX6 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Purified
Species Reactivity :
Human
Uniprot #:
P30041