ERp57 Antibody / PDIA3

NSJ Bioreagents
Product Code: NSJ-F54794
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54794-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54794-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Protein disulfide-isomerase A3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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IHC testing of FFPE human lung carcinoma tissue with Protein disulfide-isomerase A3 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 4
Western blot testing of human A2058 cell lysate with Protein disulfide-isomerase A3 antibody. Predicted molecular weight ~57 kDa.
3 / 4
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with Protein disulfide-isomerase A3 antibody.
4 / 4
Flow cytometry testing of human NCI-H292 cells with Protein disulfide-isomerase A3 antibody; Blue=isotype control, Green= Protein disulfide-isomerase A3 antibody.

IHC testing of FFPE human lung carcinoma tissue with Protein disulfide-isomerase A3 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of human A2058 cell lysate with Protein disulfide-isomerase A3 antibody. Predicted molecular weight ~57 kDa.
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with Protein disulfide-isomerase A3 antibody.
Flow cytometry testing of human NCI-H292 cells with Protein disulfide-isomerase A3 antibody; Blue=isotype control, Green= Protein disulfide-isomerase A3 antibody.

Further Information

Application Details :
Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunohistochemistry (FFPE): 1:50-1:100, Western blot: 1:500-1:1000
Application Note:
The stated application concentrations are suggested starting points. Titration of the Protein disulfide-isomerase A3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
PDIA3 is the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. The protein was once thought to be a phospholipase; however, it has been demonstrated that the protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and this protein mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 446-475 from the human protein was used as the immunogen for the Protein disulfide-isomerase A3 antibody.
Limitation:
This Protein disulfide-isomerase A3 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Purified
Species Reactivity :
Human
Uniprot #:
P30101