TCP1 gamma Antibody / CCT3

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NSJ Bioreagents
Product Code: NSJ-F54786
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54786-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54786-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the TCP1 gamma antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 4
IHC testing of FFPE human lung carcinoma tissue with TCP1 gamma antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 4
Western blot testing of human 1) MCF7, 2) CCRF-CEM, 3) K562, 4) HL60 and 5) HeLa cell lysate with TCP1 gamma antibody. Predicted molecular weight ~61 kDa.
3 / 4
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with TCP1 gamma antibody.
4 / 4
Flow cytometry testing of human K562 cells with TCP1 gamma antibody; Blue=isotype control, Green= TCP1 gamma antibody.
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IHC testing of FFPE human lung carcinoma tissue with TCP1 gamma antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of human 1) MCF7, 2) CCRF-CEM, 3) K562, 4) HL60 and 5) HeLa cell lysate with TCP1 gamma antibody. Predicted molecular weight ~61 kDa.
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with TCP1 gamma antibody.
Flow cytometry testing of human K562 cells with TCP1 gamma antibody; Blue=isotype control, Green= TCP1 gamma antibody.

Documents

Further Information

Application Details :
Flow cytometry: 1:25 (1x10e6 cells), Immunohistochemistry (FFPE): 1:25, Western blot: 1:500-1:1000
Application Note:
The stated application concentrations are suggested starting points. Titration of the TCP1 gamma antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
CCT3 is a molecular chaperone that is member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 298-326 from the human protein was used as the immunogen for the TCP1 gamma antibody.
Limitation:
This TCP1 gamma antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Purified
Species Reactivity :
Human
Uniprot #:
P49368