PIP4K2 alpha Antibody / PIP4K2A

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NSJ Bioreagents
Product Code: NSJ-F54749
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54749-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54749-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the PIP4K2 alpha antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

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IHC testing of FFPE human brain tissue with PIP4K2 alpha antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 4
Immunofluorescent staining of fixed and permeabilized human HeLa cells with PIP4K2 alpha antibody (green) and anti-Actin (red).
3 / 4
Western blot testing of human brain tissue lysate with PIP4K2 alpha antibody. Predicted molecular weight ~46 kDa.
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Flow cytometry testing of human HeLa cells with PIP4K2 alpha antibody; Blue=isotype control, Green= PIP4K2 alpha antibody.
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IHC testing of FFPE human brain tissue with PIP4K2 alpha antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of fixed and permeabilized human HeLa cells with PIP4K2 alpha antibody (green) and anti-Actin (red).
Western blot testing of human brain tissue lysate with PIP4K2 alpha antibody. Predicted molecular weight ~46 kDa.
Flow cytometry testing of human HeLa cells with PIP4K2 alpha antibody; Blue=isotype control, Green= PIP4K2 alpha antibody.

Documents

Further Information

Application Details :
Flow cytometry: 1:25 (1x10e6 cells), Immunohistochemistry (FFPE): 1:25, Western blot: 1:500-1:2000, Immunofluorescence: 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the PIP4K2 alpha antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Catalyzes the phosphorylation of phosphatidylinositol 5- phosphate (PtdIns5P) on the fourth hydroxyl of the myo-inositol ring, to form phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). May exert its function by regulating the levels of PtdIns5P, which functions in the cytosol by increasing AKT activity and in the nucleus signals through ING2. May regulate the pool of cytosolic PtdIns5P in response to the activation of tyrosine phosphorylation. May negatively regulate insulin- stimulated glucose uptake by lowering the levels of PtdIns5P. May be involved in thrombopoiesis, and the terminal maturation of megakaryocytes and regulation of their size.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 329-363 from the human protein was used as the immunogen for the PIP4K2 alpha antibody.
Limitation:
This PIP4K2 alpha antibody is available for research use only.
Localization:
Cytoplasmic, nuclear
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
P48426