UQCRFS1 Antibody

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NSJ Bioreagents
Product Code: NSJ-F54355
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54355-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54355-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the UQCRFS1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 5
IHC testing of FFPE human rectum tissue with UQCRFS1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 5
Immunofluorescent staining of fixed and permeabilized human HeLa cells with UQCRFS1 antibody (green) and DAPI nuclear stain (blue).
3 / 5
Western blot testing of human HeLa cell lysate with UQCRFS1 antibody. Predicted molecular weight ~30 kDa.
4 / 5
Western blot testing of human Raji cell lysate with UQCRFS1 antibody. Predicted molecular weight ~30 kDa.
5 / 5
Flow cytometry testing of fixed and permeabilized human HeLa cells with UQCRFS1 antibody; Blue=isotype control, Green= UQCRFS1 antibody.
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IHC testing of FFPE human rectum tissue with UQCRFS1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of fixed and permeabilized human HeLa cells with UQCRFS1 antibody (green) and DAPI nuclear stain (blue).
Western blot testing of human HeLa cell lysate with UQCRFS1 antibody. Predicted molecular weight ~30 kDa.
Western blot testing of human Raji cell lysate with UQCRFS1 antibody. Predicted molecular weight ~30 kDa.
Flow cytometry testing of fixed and permeabilized human HeLa cells with UQCRFS1 antibody; Blue=isotype control, Green= UQCRFS1 antibody.

Documents

Further Information

Application Details :
Immunofluorescence: 1:25,Flow cytometry: 1:25 (1x10e6 cells),Western blot: 1:500-1:2000,Immunohistochemistry (FFPE): 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the UQCRFS1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis. The transit peptide of the Rieske protein seems to form part of the bc1 complex and is considered to be the subunit 11/IX of that complex (By similarity).
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 181-209 from the human protein was used as the immunogen for the UQCRFS1 antibody.
Limitation:
This UQCRFS1 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
P47985