Alcohol dehydrogenase Antibody / ADH1B

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NSJ Bioreagents
Product Code: NSJ-F54429
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54429-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54429-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the ADH1B antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 5
Western blot testing of human 1) liver and 2) Jurkat cell lysate with ADH1B antibody. Predicted molecular weight ~40 kDa.
2 / 5
Western blot testing of human HepG2 cell lysate with ADH1B antibody. Predicted molecular weight ~40 kDa.
3 / 5
Immunofluorescent staining of human HepG2 cells with ADH1B antibody (green) and DAPI nuclear stain (blue).
4 / 5
IHC testing of FFPE human hepatocarinoma tissue with ADH1B antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
5 / 5
Flow cytometry testing of human HepG2 cells with ADH1B antibody; Blue=isotype control, Green= ADH1B antibody.
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Western blot testing of human 1) liver and 2) Jurkat cell lysate with ADH1B antibody. Predicted molecular weight ~40 kDa.
Western blot testing of human HepG2 cell lysate with ADH1B antibody. Predicted molecular weight ~40 kDa.
Immunofluorescent staining of human HepG2 cells with ADH1B antibody (green) and DAPI nuclear stain (blue).
IHC testing of FFPE human hepatocarinoma tissue with ADH1B antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of human HepG2 cells with ADH1B antibody; Blue=isotype control, Green= ADH1B antibody.

Documents

Further Information

Application Details :
Immunofluorescence: 1:25,Flow cytometry: 1:25 (1x10e6 cells),Immunohistochemistry (FFPE): 1:25,Western blot: 1:500-1:2000
Application Note:
The stated application concentrations are suggested starting points. Titration of the ADH1B antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
The protein is a member of the alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. This encoded protein, consisting of several homo- and heterodimers of alpha, beta, and gamma subunits, exhibits high activity for ethanol oxidation and plays a major role in ethanol catabolism.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 209-237 from the human protein was used as the immunogen for the ADH1B antibody.
Limitation:
This ADH1B antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
SAS precipitation
Species Reactivity :
Human
Uniprot #:
P00325