FAK Antibody / Focal Adhesion Kinase

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NSJ Bioreagents
Product Code: NSJ-F54458
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-F54458-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54458-0.4ML0.4 ml£502.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Aliquot the Focal Adhesion Kinase antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

1 / 5
Western blot testing of human T47-D cell lysate with Focal Adhesion Kinase antibody. Predicted molecular weight ~119 kDa.
2 / 5
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with Focal Adhesion Kinase antibody.
3 / 5
Immunofluorescent staining of human HeLa cells with Focal Adhesion Kinase antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
4 / 5
IHC testing of FFPE human brain tissue with Focal Adhesion Kinase antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
5 / 5
Flow cytometry testing of human K562 cells with Focal Adhesion Kinase antibody; Blue=isotype control, Green= Focal Adhesion Kinase antibody.
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Western blot testing of human T47-D cell lysate with Focal Adhesion Kinase antibody. Predicted molecular weight ~119 kDa.
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with Focal Adhesion Kinase antibody.
Immunofluorescent staining of human HeLa cells with Focal Adhesion Kinase antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
IHC testing of FFPE human brain tissue with Focal Adhesion Kinase antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of human K562 cells with Focal Adhesion Kinase antibody; Blue=isotype control, Green= Focal Adhesion Kinase antibody.

Documents

Further Information

Application Details :
Immunofluorescence: 1:25,Flow cytometry: 1:25 (1x10e6 cells),Immunohistochemistry (FFPE): 1:25,Western blot: 1:500-1:2000
Application Note:
The stated application concentrations are suggested starting points. Titration of the Focal Adhesion Kinase antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
FAK1/PTK2 is a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. This protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 396-423 from the human protein was used as the immunogen for the Focal Adhesion Kinase antibody.
Limitation:
This Focal Adhesion Kinase antibody is available for research use only.
Purity:
SAS precipitation
Species Reactivity :
Human
Uniprot #:
Q05397